Sasaki's Laboratory (佐々木研究室） Last up date2012/ 1/ 7
生物有機合成化学分野は2012年4月に創設１０周年を迎えます。
-Research Policy of Sasaki's Laboratory-
The research activities of Sasaki group have focused on the creation of functionalmoleculesthatexhibit specific recognition of and reaction to the DNA duplex.
Keywords: Organic Synthesis, Nucleic Acid Chemistry, Bioorganic Chemistry.OligonucleotideTherapeutics.
-Research Topics-
| ■ Naturally-Occurring Molecules-Inspired Design of New DNA-Binding Molecules (抗腫瘍活性天然物の全合成とDNA結合分子への展開) ■ Design of Intelligent Molecules with HighlySerectiveReactivityand their Incorporation into theOligonucleotides (Functionality-Transfer or Cross-linking Oligonucleotides) (インテリジェント人工核酸の設計 （官能基転移核酸・クロスリンク核酸）) ■ Development of Oligonucleotide Therapeutics (インテリジェント人工核酸のDNA医薬品への展開) ■ Expansion of Triplex Recognition Codes with W-shapedNucleoside Analogs (WNA) (3本鎖形成人工核酸の開発（W-字型人工核酸）) ■8-OxoG-clamp as a Selective Fluorescence Quenching Probe for8-Oxo-Deoxyguanosine "遺伝子損傷部位を特異的に認識する分子の開発 （8オキソグアノシン認識分子）) |
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2011年4月 能古島アイランドパーク
●大学院生募集
佐々木研では、博士研究員、博士課程、修士課程の学生を多数募集しています。また、研究室見学も大歓迎です。興味のある方は、連絡をお待ちしております。
●当研究室所有の機器の一部を紹介します。
What's New
2012.1.7.
■News を up しました。
2011.12.14.
■News を up しました。
■12月10日 修士2年 渕靖史さんが薬学会九州支部大会で優秀発表賞を受賞しました。
2011.11.30.
■News、Publication を up しました。
■Sasaki S., Onizuka K., Taniguchi Y., Oligonucleotide containing S-Functionalized 2'-deoxy-6-thioguanosine:Facile Tools for Base-Selective and Site Specific Internal Modification of RNA, Current Protocols, accepted.
2011.11.15.
■News、Publication を up しました。
■Taniguchi Y, Koga Y, Fukabori R, Kawaguchi R, Sasaki S., OFF-to-ON type fluorescent probe for the detectionof 8-oxo-dG in DNA by the Adap-masked ODN probe, Bioorg. Med. Chem. Lett. (2011) in press
We have recently reportedthat Adap (Adenosine-1,3-diazaphenoxazine) is an artificial nucleoside analoguefor the specific recognition by multiple hydrogen bonding and that itsfluorescence is selectively quenched with 8-oxo-2’-deoxyguanosine (8-oxo-dG) inDNA. We now report the development of a new OFF-to-ON type FRET probe, in whichone strand contains Adap and another contains natural nucleotides for theformation of a less stable double strand. Each strand was labeled with Cy3 orBHQ2 at the 5’-end or 3’-end, respectively. It was expected in this system thatfluorescence of the duplex probe is first quenched by FRET, but the target DNAstrand containing 8-oxo-dG at the complementary site of Adap would enhance thedisplacement reaction of the less stable duplex probe that results in thefluorescence recovery. The results showed that the duplex probe containing theAdap-T base pair exhibited a complete discrimination between 8-oxo-dG and dG inDNA by fluorescence enhancement.
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